数据质控报告

1 测序reads统计

测序reads统计如下表：

表1 测序数据统计

Type	Values
Number of Reads	XXX
Valid Barcodes	XXX
Valid UMIs	XXX

注：
Number of Reads：reads总数；
Valid Barcodes：包含有效Barcode的reads数；
Valid UMIs：包含有效UMIs的reads数。

2 数据比对

比对结果统计如下表：

表2 比对结果统计

Type	Values
Reads Mapped to Genome	XXX
Reads Mapped Confidently to Genome	XXX
Reads Mapped Confidently to Intergenic Regions	XXX
Reads Mapped Confidently to Intronic Regions	XXX
Reads Mapped Confidently to Exonic Regions	XXX
Reads Mapped Confidently to Transcriptome	XXX

注：
Reads Mapped to Genomes：比对到参考基因组上的Reads在总Reads中占的比例；
Reads Mapped Confidently to Genome：比对到参考基因组并得到转录本GTF信息支持的Reads在总Reads中占的比例；
Reads Mapped Confidently to Intergenic Regions：比对到基因间区域的Reads在总Reads中占的比例；
Reads Mapped Confidently to Intronic Regions：比对到内含子区域的Reads在总Reads中占的比例；
Reads Mapped Confidently to Exonic Regions：比对到外显子区域的Reads在总Reads中占的比例；
Reads Mapped Confidently to Transcriptome：比对到已知参考转录本的Reads在总Reads中占的比例。

3 分析统计

Cell分析统计如下：

表3 Cell统计

Type	Values
Estimated Number of Cells	XXX
Median UMI Counts per Cell	XXX
Median Genes per Cell	XXX
Total Genes Detected	XXX
Sequencing Saturation	XXX
Fraction Reads in Cells	XXX

注：
Estimated Number of Cells：检测到的细胞数；
Median UMI Counts per Cell：每个cell的UMI中位数；
Median Genes per Cell：每个cell中基因的中位数；
Total Genes Detected：基因总数；
Sequencing Saturation：测序饱和度；
Fraction Reads in Cells：过滤后细胞reads数占总reads数比例。

图2 Barcode rank统计图