Product Overview
The BMKMANU S-series spatial transcriptomics chip, independently developed by BMKMANU, is a microwell-and-bead-based subcellular-resolution spatial transcriptomics product with a maximum resolution of 4.8 um. It pioneers the combination of on-slide H&E staining, on-slide fluorescence imaging and on-slide high-throughput sequencing for accurate cell segmentation, enabling precise single-cell-level spatial transcriptomics sequencing and greatly advancing basic research. It is now widely applied in developmental diseases, physiological disorders, embryonic development, stem cell differentiation, 3D mapping, reproductive health, oncology and other fields.
Principle of BMKMANU S-series cell segmentation: animal samples
How BMKMANU S-series spatial transcriptomics cell segmentation works: three images in one
Building on the underlying design of the S-series spatial chip, BMKMANU has developed its own patented technology that yields error-free on-slide fluorescence images and H&E images perfectly aligned with the chip. Nuclei are localized and their boundaries delineated from the fluorescence image; the H&E image provides calibration to help delineate cell boundaries. Boundary information is mapped onto the chip, the sequencing data at the corresponding positions are extracted, and accurate single-cell segmentation is achieved.
Accurate cell segmentation by combining three images in BMKMANU S-series
Case Studies of BMKMANU S-series Cell Segmentation
Animal Experiments
Mouse brain cell segmentation: Number of Cells 75,101; Median Genes per Cell 1,568
Demo data, mouse olfactory bulb: Number of Cells 17,564; Median Genes per Cell 1,142
Demo data, mouse testis: Number of Cells 137,671; Median Genes per Cell 645
Principle of BMKMANU S-series cell segmentation: plant samples
Compared with animal cells, plant cells vary enormously in shape and size; applying a single cell size for prediction would produce poor segmentation. To better match the characteristics of plant samples and support plant research, BMKMANU applies multi-scale progressive prediction to plant cells. Combined with BMKMANU's general plant segmentation model, it can, within a single plant tissue section, ... to predict and segment cells of different sizes, greatly improving the accuracy of plant cell segmentation.
Multi-scale cell segmentation for plant tissue in BMKMANU S-series
Case Studies of BMKMANU S-series Cell Segmentation
Plant Experiments
Companion Software
BMKMANU also provides free Windows visualization software, BSTCellViewer (free download from the BMKMANU website). It combines the output of the BMKMANU plant multi-scale segmentation pipeline with high-definition on-slide brightfield images, allowing manual editing, removal and outlining of cells for more accurate results.
