Product Overview

BMKMANU DG1000 uses droplet encapsulation and barcoding to achieve high-throughput cell capture. The water-in-oil process is completed in as little as 110 s. The instrument is compact and portable, and its BMKMANU C1000 chip has independent 2 x 4 channels that can be flexibly adjusted according to sample number. The product also comes with the companion analysis software BSCMatrix.

BMKMANU DG1000 Advantages

Compact and Portable

Easy to Use On-Site

Fast Operation

Droplet generation completed within 110 s

Flexible Sample Loading

Independent 2 x 4 chip

Compatible with Larger Cell Diameters

Successfully supports cells up to 60 um

Workflow

Capture Probe

There are approximately 880,000 probe types, with tens of millions of probes on each gel bead. Each probe consists of four parts: the universal sequencing primer R1, the cell barcode, the UMI and poly(dT)VN. Here we designed a longer cell barcode of 54 bp, which improves compatibility with long-read sequencing, especially ONT sequencing, and increases identification efficiency.

Outsourced Service vs. In-House Operation

Single-Cell Sequencing Workflow	Outsourced Service	In-House
Experiment Turnaround	〜10d	1〜2d
Analysis Turnaround	〜10d	1〜2d
Shipping Required	Shipping takes 1-2 days and may reduce cell viability	No shipping; dissociation on site, preserving high cell viability

Short Turnaround

An outsourced service takes about 20 days, while in-house library construction plus data analysis takes only 2-4 days.

Preserved Cell Viability

For precious samples, single-cell suspensions are prepared on site, avoiding the loss of viability caused by long-distance shipping.

Lower Cost

Lower cost, and you can perform downstream data analysis yourself, saving both time and reagent/consumable costs.

Benchmark Data

1. Sequencing Reads Statistics

Sequencing reads statistics are shown below:

Table1 Sequencing Data Statistics

Type	Values 221020-4-2	Values 21020-8-7-1
Number of Reads	399,967,594	378,584,610
Valid Barcodes	339,047,468	323,897,836
Valid UMIs	395,738,572	374,651,123

Note:
Number of Reads: total number of reads;
Valid Barcodes: number of reads containing a valid barcode;
Valid UMIs: number of reads containing a valid UMI.

2. Read Alignment

Alignment statistics are shown below:

Table 2 Alignment Statistics

Type	Values 221020-4-2	Values 21020-8-7-1
Reads Mapped to Genome	96.01%	95.52%
Reads Mapped Confidently to Genome	80.77%	81.7%
Reads Mapped Confidently to Intergenic Regions	1.69%	2.58%
Reads Mapped Confidently to Intronic Regions	15.62%	29.15%
Reads Mapped Confidently to Exonic Regions	63.44%	49.96%
Reads Mapped Confidently to Transcriptome	71.64%	71.57%

Note:

Reads Mapped to Genomes: proportion of reads mapped to the reference genome among total reads;

Reads Mapped Confidently to Genome: proportion of reads confidently mapped to the reference genome and supported by transcript GTF information;

Reads Mapped Confidently to Intergenic Regions: proportion of reads confidently mapped to intergenic regions;

Reads Mapped Confidently to Intronic Regions: proportion of reads confidently mapped to intronic regions;

Reads Mapped Confidently to Exonic Regions: proportion of reads confidently mapped to exonic regions;

Reads Mapped Confidently to Transcriptome: proportion of reads confidently mapped to known reference transcripts.

3. Analysis Statistics

Cell analysis statistics are shown below:

Table3 CellStatistics

Type	Values 221020-4-2	Values 21020-8-7-1
Estimated Number of Cells	7,104	11,012
Median UMI Counts per Cell	7,261	6,434
Median Genes per Cell	2,624	2,678.5
Total Genes Detected	37,416	41,208
Sequencing Saturation	73.22%	65.99%
Fraction Reads in Cells	77.39%	83.64%

Note:
Estimated Number of Cells: number of cells detected;
Median UMI Counts per Cell: median UMI count per cell;
Median Genes per Cell: median gene count per cell;
Total Genes Detected: total number of genes;
Sequencing Saturation: sequencing saturation;
Fraction Reads in Cells: proportion of reads in cells after filtering relative to total reads.

Figure 2. Barcode rank plot for 221020-4-2

Figure 2. Barcode rank plot for 21020-8-7-1

BMKMANU DG1000 single-cell microdroplet platform