Overview of S3000-FFPE Spatial Transcriptomics Platform
FFPE samples, the most prevalent sample type in clinical pathology, harbor invaluable biological information. However, inherent RNA degradation and protein-nucleic acid crosslinking induced by formalin fixation create substantial barriers to unlocking their rich molecular information.
BMKMANU S3000-FFPE spatial transcriptomics platform innovatively adopts a proprietary poly(A) capture technology together with a patented de-crosslinking solution, precisely overcoming the key challenges of RNA degradation and protein-nucleic acid crosslinking. With high specificity and capture efficiency for mRNA , it offers an effective approach for whole transcriptome profiling of FFPE samples across diverse species.
Challenges of Spatial Transcriptomics for FFPE Samples
Severe RNA Degradation
Formalin fixation causes nucleic acid breakage and cross-linking. Long-term storage exacerbates fragmentation
Limitations of Probe Capture Method
Pre-designed probe panels with only ~18,085 target genes, which constrains de-novo discoveries.
Limitations of Random Primer Method
Bind to total RNA, reducing mRNA capture efficiency. Low-abundance mRNAs may be missed.
Advantages of S3000-FFPE Spatial Transcriptomics Platform
No Species Limitation • Whole mRNA Capture
High Specificity • High Sensitivity
Patented de-crosslinking technology
BMKMANU proprietary decrosslinking technology for FFPE samples to maximize mRNA release.
Sub-cellular Resolution
Workflow of S3000-FFPE Spatial Transcriptomics Platform
Case Study for S3000-FFPE Spatial Transcriptomics Platform
This platform has been validated across multiple species, including human, mouse, rhesus macaque, and salamander, as well as diverse tissue types, encompassing skin, bladder, and esophageal cancer. The results show high stability and reproducibility.
01 • Sample Overview
02 • Sample Details
03 • Representative Cases (Cell Segmentation)
