The Perfect Pair! An Indispensable Golden Combination in Spatiotemporal Omics!

By technical principle, spatial transcriptomics technologies fall into three major categories: those based on microdissection (LCM-RNA), which sequence minute local regions by microdissecting the sample; those based on high-throughput sequencing (NGS), which encode positional information into transcripts (for example, the BMKManu S3000 spatial product); and third, image-based methods, including in situ hybridization (ISH)-based approaches (imaging probes are sequentially hybridized in the tissue) and in situ sequencing (ISS)-based approaches (transcripts are amplified and sequenced directly in the tissue). Fluorescence in situ hybridization (FISH, FISH) can specifically and sensitively reveal the spatial distribution of gene expression at the single-cell level, and has therefore long served as the go-to “validation partner” for single-cell or spatial transcriptomics sequencing.

Classification of spatial transcriptomics technologies

To serve the wider research community, BMK Manufacturing keeps innovating and has officially launchedBMKMANU ISSeq Kit, dedicated to supporting scientific research.

BMK RNA in situ fluorescence:BMKMANU ISSeq Kit

The BMK RNA in situ fluorescence kit is based on advanced padlock probes, fluorescent probes and rolling circle amplification technology. It can target and capture target RNA and, using rolling circle amplification products as the mediator, achieve in situ fluorescent visualization of RNA directly in tissue or cells. This technology ensures precise capture of RNA localization within tissues or cells at the single-cell level. It is the ideal partner for downstream validation of single-cell sequencing and spatial transcriptomics, providing researchers with more reliable and more precise experimental results.

Product features

Sample-friendly, outstanding quality: designed specifically for fresh OCT-embedded samples, perfectly preserving tissue structure, reducing shrinkage and deformation, and ensuring reliable, reproducible results. Sample preparation is fast and simple, greatly improving experimental efficiency.

Cross-species compatibility, boundless research: broadly applicable to animals and plants — whether exploring mammalian physiological mechanisms or dissecting plant gene expression patterns, it handles both with ease. Cross-species research helps reveal the commonalities of life and promotes interdisciplinary integration.

Flexible gene panels, customized as you wish: panels can be freely adjusted to contain 1–10 genes to meet individualized research needs. Precise design and simultaneous detection of multiple genes make research more efficient and more accurate.

Precise and reliable, worry-free validation: rigorously validated experimentally and benchmarked against the “gold standard,” it demonstrates outstanding accuracy. In the case studies, imaging and cell segmentation of multiple tissue samples, including the mouse olfactory bulb, precisely present the spatial distribution of genes, in high agreement with authoritative database results.

Selected product results

Mouse olfactory bulb

A panel was designed for six genes — “Gsn, Slc17a7, Gad1, Fabp7, Nr2f2, Penk” — and imaging together with the corresponding cell segmentation was performed, precisely showing the spatial distribution of these genes. A one-to-one comparison with the corresponding FISH results provided by the Allen Brain Atlas website showed excellent agreement, demonstrating the outstanding accuracy of the BMK RNA in situ fluorescence kit.

The BMK RNA in situ fluorescence hybridization product supports expression analysis of panel genes:

Mouse brain

Mouse testis

Human breast cancer

Mouse kidney

Since the launch of its spatial products (BMKMANU S1000) in April 2022, BMK Manufacturing has continued to invest in the spatial field, successively releasing BMKMANU S2000, BMKMANU S3000, BMKMANU 5000, as well as the precision spatial imaging system BH1000 and spatial immune repertoire products. Now, the BMK RNA in situ fluorescence hybridization kit has also arrived. BMK Manufacturing has always focused on the R&D of spatiotemporal omics-related products and instruments, committed to opening up more possibilities for the life sciences.