Applicable to frozen tissue sections derived from plant and animal samples, all of which are compatible with sequencing. Each capture area contains approximately 2 million barcoded mRNA capture spots, with an average of 0.25 cells captured per spot.

With an improved microfluidic channel design, the reaction speed is enhanced and the assay time is shortened. The entire experimental reaction can be completed in less than two minutes, reducing environmental stress on cell states and thereby enabling higher-quality single-cell data.

Leveraging the ultra-long reads of the ONT platform and the longer spatial barcodes of S1000, downstream data demultiplexing can be performed without relying on NGS data.

Fluorescence-based nuclear localization is used to delineate nuclear boundaries, while H&E-based registration assists in delineating cell boundaries, enabling accurate single-cell segmentation.