BMKMANU S1000空间转录组细胞分割Demo数据

百创智造S系列空间转录组细胞分割Demo下载
2023年7月21日
Bmk_Space_mapping.R Operating Process
2023年9月19日

数据相关统计

表1 测序Reads统计

Type Number Percent(%)
Number of Reads 337,531,256
Valid Barcodes 316,152,592 93.67
Valid UMIs 335,911,164 99.52
Final Valid Reads 316,136,080 93.66

表2 数据比对

Type Percent(%)
Reads Mapped to Genome 97.38%
Reads Mapped Confidently to Genome 84.94%
Reads Mapped Confidently to Intergenic Regions 1.64%
Reads Mapped Confidently to Intronic Regions 2.76%
Reads Mapped Confidently to Exonic Regions 80.53%
Reads Mapped Confidently to Transcriptome 79.15%

Spots相关统计

Summary组织切片HE染色图组织UMI count统计图
表3 Spots统计
Tpyes Values
Sequencing Saturation 87.97%
Percent of Spots Under Tissue 11.00%
Fraction Reads in Spots Under Tissue 91.21%
表4 不同水平Spots统计
Level 13 (100μm) 7 (50μm) 6 (42μm) 5 (35μm) 4 (27μm) 3 (20μm) 2 (10μm) 1 (5μm)
Number of SupSpots 562 2,075 2,888 4,310 7,102 13,851 37,548 213,000
Median Genes per SupSpot 9,082 4,841 3,962 3,053 2,137 1,284 551 111
Median UMI Counts per SupSpot 54,596 14,645 10,474 6,998 4,221 2,131 767 130
Total Genes Detected 29,175 29,170 29,182 29,181 29,175 29,191 29,190 29,196

图1 组织切片HE染色图

图2 组织UMI count统计图

聚类分析

L13 cluster聚类图L7 cluster聚类图L5 cluster聚类图

图3 cluster聚类图

图3 cluster聚类图

图3 cluster聚类图

细胞分割

Summary组织切片荧光图组织切片HE分割图组织切片荧光分割图细胞分割聚类图
表5 细胞分割统计
Type Value
Number of Cells 17,567
Median Genes per Cells 1,165
Median UMI Counts per Cells 1,786
Total Genes Detected 29,059

图4 组织切片荧光图

图5 组织切片HE分割图

图6 组织切片荧光分割图

图7 细胞分割聚类图