BMKManu DG1000 Human PBMC Sequencing Data Details

Sequencing read statistics

Sequencing read statistics are shown in the table below:

Notes:
Number of Reads: total number of reads;
Valid Barcodes: number of reads containing a valid barcode;
Valid UMIs: number of reads containing valid UMIs.

Read alignment

Alignment statistics are shown in the table below:

Notes:
Reads Mapped to Genomes: proportion of reads aligned to the reference genome out of total reads;
Reads Mapped Confidently to Genome: proportion of reads aligned to the reference genome and supported by transcript GTF information;
Reads Mapped Confidently to Intergenic Regions: proportion of reads aligned to intergenic regions;
Reads Mapped Confidently to Intronic Regions: proportion of reads aligned to intronic regions;
Reads Mapped Confidently to Exonic Regions: proportion of reads aligned to exonic regions;
Reads Mapped Confidently to Transcriptome: proportion of reads aligned to known reference transcripts.

Analysis statistics

Cell analysis statistics are as follows:

Notes:
Estimated Number of Cells: number of detected cells;
Median UMI Counts per Cell: median UMI count per cell;
Median Genes per Cell: median number of genes per cell;
Total Genes Detected: total number of genes;
Sequencing Saturation: sequencing saturation;
Fraction Reads in Cells: proportion of reads from cells after filtering out of total reads.

Barcode rank plot