某动物皮肤单细胞Demo数据

BSTMatrix软件使用说明
BSCMatrix v1.9环境搭建及使用
2022年7月15日
S1000 Seurat分析流程
2022年8月3日

测序reads统计

测序reads统计如下表:

Type Values
Number of Reads 187088582
Valid Barcodes 162097865
Valid UMIs 175241433

注:
Number of Reads:reads总数;
Valid Barcodes:包含有效Barcode的reads数 ;
Valid UMIs:包含有效UMIs的reads数。

数据比对

比对结果统计如下表:

Type Values
Reads Mapped to Genome 89.80%
Reads Mapped Confidently to Genome 72.68%
Reads Mapped Confidently to Intergenic Regions 5.79%
Reads Mapped Confidently to Intronic Regions 6.20%
Reads Mapped Confidently to Exonic Regions 60.68%
Reads Mapped Confidently to Transcriptome 54.81%

注:
Reads Mapped to Genomes:比对到参考基因组上的Reads在总Reads中占的比例;
Reads Mapped Confidently to Genome:比对到参考基因组并得到转录本GTF信息支持的Reads在总Reads中占的比例;
Reads Mapped Confidently to Intergenic Regions:比对到基因间区域的Reads在总Reads中占的比例;
Reads Mapped Confidently to Intronic Regions:比对到内含子区域的Reads在总Reads中占的比例;
Reads Mapped Confidently to Exonic Regions:比对到外显子区域的Reads在总Reads中占的比例;
Reads Mapped Confidently to Transcriptome:比对到已知参考转录本的Reads在总Reads中占的比例。

分析统计

Cell分析统计如下:

Type Values
Estimated Number of Cells 3376
Median UMI Counts per Cell 5504.5
Median Genes per Cell 2069.5
Total Genes Detected 26262
Sequencing Saturation 76.38%
Fraction Reads in Cells 87.72%

注:
Estimated Number of Cells:检测到的细胞数;
Median UMI Counts per Cell:每个cell的UMI中位数;
Median Genes per Cell:每个cell中基因的中位数;
Total Genes Detected:基因总数;
Sequencing Saturation:测序饱和度;
Fraction Reads in Cells:过滤后细胞reads数占总reads数比例。

 Barcode rank统计图

Barcode rank统计图