BSCMatrix v1.9 Environment Setup and User Guide
2022-07-15
S1000 Seurat Analysis Pipeline
2022-08-03

Sequencing read statistics

Sequencing read statistics are shown in the table below:

[su_table]

Type Values
Number of Reads 187088582
Valid Barcodes 162097865
Valid UMIs 175241433

[/su_table]

Notes:
Number of Reads: total number of reads;
Valid Barcodes: number of reads containing a valid barcode;
Valid UMIs: number of reads containing valid UMIs.

Read alignment

Alignment statistics are shown in the table below:

[su_table]

Type Values
Reads Mapped to Genome 89.80%
Reads Mapped Confidently to Genome 72.68%
Reads Mapped Confidently to Intergenic Regions 5.79%
Reads Mapped Confidently to Intronic Regions 6.20%
Reads Mapped Confidently to Exonic Regions 60.68%
Reads Mapped Confidently to Transcriptome 54.81%

[/su_table]

Notes:
Reads Mapped to Genomes: proportion of reads aligned to the reference genome out of total reads;
Reads Mapped Confidently to Genome: proportion of reads aligned to the reference genome and supported by transcript GTF information;
Reads Mapped Confidently to Intergenic Regions: proportion of reads aligned to intergenic regions;
Reads Mapped Confidently to Intronic Regions: proportion of reads aligned to intronic regions;
Reads Mapped Confidently to Exonic Regions: proportion of reads aligned to exonic regions;
Reads Mapped Confidently to Transcriptome: proportion of reads aligned to known reference transcripts.

Analysis statistics

Cell analysis statistics are as follows:

[su_table]

Type Values
Estimated Number of Cells 3376
Median UMI Counts per Cell 5504.5
Median Genes per Cell 2069.5
Total Genes Detected 26262
Sequencing Saturation 76.38%
Fraction Reads in Cells 87.72%

[/su_table]

Notes:
Estimated Number of Cells: number of detected cells;
Median UMI Counts per Cell: median UMI count per cell;
Median Genes per Cell: median number of genes per cell;
Total Genes Detected: total number of genes;
Sequencing Saturation: sequencing saturation;
Fraction Reads in Cells: proportion of reads from cells after filtering out of total reads.

Barcode rank plot

Barcode rank plot